Rabbit polyclonal antibody

Antigen/Purification: ExpandCollapse

The antigen is a peptide from the NR1 subunit, C2 splice variant insert of rat NMDA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2′). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Storage

25 µg antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂PO₄, pH 7.4) before use. After reconstitution the antibody should be aliquoted and stored at -20° C. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Brady ML, Diaz MR, Iuso A, Everett JC, Valenzuela CF, Caldwell KK (2013) Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. J Neurosci. 33(3):1062-7.  PMID: 23325244

Mannie M. Y. Fan, Herman B. Fernandes, Lily Y. J. Zhang, Michael R. Hayden, and Lynn A. Raymond (2007) Altered NMDA Receptor Trafficking in a Yeast Artificial Chromosome Transgenic Mouse Model of Huntington’s DiseaseJ. Neurosci., 27: 3768 – 3779.  PMID: 17409241