The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies [1]. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK Tcells. Primary rat splenocytes (RS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.
RS from ScienCell Research Laboratories are isolated from normal adult CD® IGS rat spleen. RS are depleted of splenic macrophages, cryopreserved directly after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. RS are quality control tested for viability. RS are negative for mycoplasma, bacteria, yeast, and fungi. RS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.
Specifications:
| Catalog No. | R5540 |
| Country of Manufacture | United States |
| Product Code | RS |
| Size/Quantity | 10 million cells in 1 ml volume |
| Product Use | RS are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Mebius R, Kraal G. (2005) \"Structure and function of the spleen.\" Nat Rev Immunol 5(8): 606-616. |
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